There is a dearth of information on biosecurity practices in major fish farms in Ekiti State. With the rising activities of fish farming in the State, it is imperative to know the biosecurity measures been practiced by farmers to protect their farms. The study identified, evaluated and compared different biosecurity measures in eighty (80) randomly selected farms across eight (08) Local Government Areas (LGA) of the State at ten (10) farms per LGA.
Primary data were generated through validated questionnaires, w hile reliable secondary data were sourced from both the State and Federal Ministries of Agriculture. Data were analyzed using frequency, percentage counts and pie charts. 50% of the farm sites were fenced, either with blocks or wire gauze, while 30% had security Dogs. Only 5% place disinfectants as dip at farm entrance. Less than 10% disallowed outsiders from entering into the main facilities of the farms, while 5% engaged in the use of voodoo (African magic) to secure their farms . 40% of the farmers engaged the services of security men. The study revealed that unchecked human traffic in and out of farms led to high mor tality of fish, with occasional disappearance of farm tools. H igh percentage of hired security men did not translate to better protection of farms compared to the use of security dogs and voodoo. Fencing and installation of anti-birds nets are necessary. It is recommended that outsiders should be disallowed from entering into the farms while the use of non-human security measures should be encouraged.