Most of the farmers were males (71.7%), aged > 50 years (51.7%), and (38.3%) were university graduates, with (80%) having no training in aquaculture, and 50% having no access to extension services. The majority were aware of antibiotics (80%), and antimicrobial resistance (93%). However, only 35% of the farmers had a positive attitude towards antimicrobial agents. Although none of the farmers indicated using antibiotics, we noted pond fertilization with manure in 38.3% of the farms. A total of 84 farmed fish were sampled and analysed for tetracycline and sulphonamide residues, of which 100% (n = 84) were positive for sulphonamide residues; 2.38% (n = 2) were positive for tetracycline and 2.38% (n = 2) contained both sulphonamide and tetracycline residues. The concentration of the residues ranged from 0 to 6.95 µg/kg for tetracycline, and 12.68 to 133 µg/kg for sulphonamides, respectively. About 5.95 % (n=5) of the samples had sulphonamide residues above the MRL, while none of the samples had tetracycline residues above the MRL. All samples that were positive for tetracycline were within the acceptable daily intake ranges, while 28.47% (n = 24) samples had sulphonamide concentrations above the acceptable daily intake range. Fish farmers in Dar es Salaam have significant gaps in KAP regarding antimicrobial use, and there is a presence of antibiotic residues in farmed fish, issues that need to be addressed.