There is a critical need to develop innovative and sustainable aquaculture technologies to expand production of seafood to meet the growing demand by consumers for safe seafood resources , to reduce fishing pressure on declining wild fish populations, and to improve the environmental sustainability of our seafood production systems. Mote Aquaculture Research Park (Mote) scientists are working together with our research partners at the University of South Florida’s College of Engineering (USF) and the National Mariculture Center (NMC) in Israel to develop integrated multi-trophic aquaculture (IMTA) or marine aquaponics farming systems to expand the availability of healthy seafood for local communities. These i ntegrated systems are engineered to efficiently use nutrients, water and energy to produce marine fish and edible sea vegetables . The integration of periphyton biofilters into the recirculating aquaculture system (RAS) improves water quality, nutrient resource recovery, microbiome diversity and water treatment efficiency. Incorporation of periphyton biofilters into the Mote IMTA not only improved water quality by reducing total ammonia nitrogen (TAN), nitrite, nitrate and carbon dioxide , but produced dissolved oxygen and repurposed nutrients that can be used as a potential ingredient for fish feeds. Some of the nutrients that were removed from the water (TAN, nitrite , etc.) can be reclaimed by harvesting from the nets once per month. Mote has developed a brackish water IMTA with a periphyton biofilter as a demonstration pilot-scale marine aquaponics farming system. This system can be managed by family or farmer-owned cooperatives , to supply communities with locally produced fresh seafood and provide expanded job opportunities in rural and urban communities.