Aquaculture Africa 2023

November 13 - 16, 2023

Lusaka, Zambia

EFFECT OF TRYPTOPHAN DIETARY CONTENT ON MEAGRE Argyrosomus regius JUVENILES' GROWTH, MUSCLE CELLULARITY AND BEHAVIOURAL RESPONSE

Margarida Saavedra1, Ana Vasconcelos1, Teresa G. Pereira1, Marisa Barata2 , Luís Conceição3 , Pedro Pousão2 and Marta  C. Soares4

1 Portuguese Institute for the Sea and Atmosphere ,  Rua Alfredo Magalhães Ramalho, nº6, 1495-006 Lisbon, Portugal

2 Aquaculture Research Station of IPMA (EPPO), Av. do Parque Natural da Ria Formosa s/n, 8700-194 Olhão, Portugal

3 Sparos Lda, Área Empresarial de Marim, Lote C, 8700-221 Olhão, Portugal

4BIOPOLIS, CIBIO-InBIO, Universidade do Porto, Vairão Portugal

 



The increasing importance of fish welfare in aquaculture is not only an ethical question but a chance to improve standards and quality of fish production technologies and aquaculture products. Fish kept under good welfare conditions have lower stress levels, are less prone to diseases, have better growth and food conversion rates (FCR) and have a better final  flesh quality. T ryptophan is known to be linked to the  stress  response by enhancing fish brain serotonergic activity and can also inhibit endogenously derived behavioural performance, such as aggressiveness and cannibalism. In this study, three diets containing different contents of tryptophan: 0.5 (Trip1), 0.7 (Trip2) and 0.8 % (Trip3), were tested in triplicates in 112 days old meagre with an initial weight of 32.6 ± 3.4 g and 14.4 ± 0.5 cm length for 57 days. Although the results showed no significant differences  for growth and FCR  between treatments , the re was a tendency to an increase of growth and decrease of FCR in meagre fed higher levels of tryptophan. This is supported by the results obtained in  muscle cellularity where Trip3  had a higher fibre density, suggesting an increased fibre recruitment. In terms of fish behaviour, very little differences were found . In the anxiety test, fish fed the  higher quantity of tryptophan showed a  significantly lower number of freezing  episodes compared to fish fed the lowest tryptophan amount , suggesting  more ability to explore and lower levels of anxiety . These results suggest that tryptophan  dietary levels can influence not only meagre growth pattern but, as well, its behavioural response.