Fish is an important PUFA source for human dietary. Many researches were focused on increasing the PUFA products of fish. Besides high intake of PUFA consumption, improving biosynthesis of PUFAs would also be one way to contribute to nutrition supplementary for human healthy. Aquaculture selective breeding by genetic markers is essential for increasing PUFAs content. Fatty acid desaturase 2 (fads2) and elongase 5 (elovl5) have been proven key rate-limiting enzymes in the synthesis of PUFAs for bony fishes. These genes have been proved for biosynthesis PUFA. However, the promoter SNPs (pSNPs) and the coding SNPs (cSNPs) effectiveness for the PUFA contents has not been evaluated yet. This study identified the genetic variants in the promoter regions and coding regions of fads2a/b and elovl5a/l5b. Moreover, the joint effects of multiple cSNPs and pSNPs in fads2b and elovl5b, two major genes related to the PUFA biosynthesis, were evidenced with the higher explained percentages of phenotype. In further, fads2b gene express level showed correlation to more PUFAs. These suggestive SNPs were used to evaluate the breeding values of PUFAs. These SNPs would be potential markers for future selection to improve the PUFA contents in common carp.
Keywords: Association study; Common carp; Fatty acid elongase; Promoter; Coding region; Poly-unsaturated fatty acid