Phospholipids are known to be components of lipoproteins which generally transport lipids, critical enhance higher amount of dietary cholesterol from digestive tract to circulatory system and especially work as emulsifier in shrimp feed. In addition, lysophospholipids are enzymatic hydrolysis of phospholipids and have a higher function of emulsifier to promote lipid utilization in shrimps. The objective of this present study is to investigate on the effect of lysophospholipids with partial replacement of fish oil on growth performance and cholesterol status.
The study is assigned in CRD with 4 treatments and three replicates. Four diets are supplemental with different levels of fish oil and lysophospholipid (Lypotech EC) in Table 1. Healthy white shrimp with initial mean weight of 1.551±0.03 gram/head are randomly 25 shrimp distributed to each aquarium of 0.53 × 1.22 × 0.37 meters (totally 12 aquariums) and the density is 140 shrimp/m3. During the 8-week feeding trial, the shrimp are fed 12 % of their body weight per day at first week and calculated feeding from 10 to 5% every 2 weeks until the end of experiment. All shrimp are fed 4 times a day at 08.00, 11.00, 15.00 and 19.00. Shrimp are weighed every 2 weeks and then collected hemolymph and meat sample for study cholesterol status. The results in Figure 1 show that shrimp fed diets of lysophospholipids in T3 and T4 have higher average daily gain (ADG), specific growth rate (SGR) and lower feed conversion ratio (FCR) follow by T1 which has higher growth performance compares to shrimp fed negative diet with 1% of fish oil (T2) (P > 0.05). The amount of cholesterol in hemolymph of shrimp fed diet with lysophospholypids (T3, T4) in Figure 2 has an increase compares to negative control diet (T2) whereas cholesterol in shrimp meat has significantly lower (P < 0.05). Therefore,
lysophospholipids 0.03 - 0.06% in fish oil reduction diets can improve growth performance and feed utilization under the proportion of 1:16-1:33.