Proteomics and metabolomics are powerful tools used to determine the effects that environmental stimuli exert upon aquaculture organisms and the importance of this for growth, immunity, and resilience. Here, proteomics and metabolomics were used to identify altered molecular pathways in response to diet in shrimp (haemolymph and hepatopancreas) while proteomics was used to monitor biomarkers in liver of heat stressed Atlantic salmon.
Here, hepatopancreas proteomics and haemolymph metabolomics were used to investigate the post-prandial response of black tiger shrimp (Penaeus monodon) fed a conventional fishmeal diet (FM); a diet supplemented with the microbial biomass NovacqTM (NV); krillmeal (KM); or, fasted (FS). NV fed shrimp showed preference for energy derived from carbohydrates indicated by a strong signature of glycoconjugate metabolism and activation of the amino- and nucleotide sugar metabolic pathway. KM activated the glyoxylate and dicarboxylate pathway that denoted shrimp preference for lipidic energy. FS shrimp displayed down-regulation of oxidative phosphorylation and resorted to internal lipid reserves for energy homeostasis displaying a strong signature of autophagy.
In Atlantic salmon Salmo salar, a 45-peptide targeted proteomics method was used to measure biomarkers for heat stress in liver of salmon exposed to gradual temperature increases from 15°C to 20°C. Abundance of two different serpin peptides significantly increased as the temperature increased to 17°C, 19°C and 20°C compared with a control group maintained at 15°C.
Combined proteomics and metabolomics revealed heterogeneity in shrimp pathway activation for energy use and identified N-acetyl D-galactosamine as a unique feature in shrimp fed NovacqTM. In salmon, two serpin peptides showed the greatest potential as heat stress biomarkers that could help monitor the effectiveness of feed additives for maintaining salmon welfare at high temperature