World Aquaculture 2023

May 29 - June 1, 2023

Darwin, Northern Territory, Australia

DIETARY SUPPLEMENTATION OF A MIXTURE OF SAPONIN-RICH PLANTS TO REDUCE AMMONIA-NITROGEN EXCRETION IN SHRIMP Penaeus vannamei

Sekhou Cissé*, Joseph Bernot, Maxime Le Bot

 

Nor-Feed SAS
3 rue Amédéo Avogadro, 49070 Beaucouzé, France

 Email: sekhou.cisse@norfeed.net

 



The intensification in aquaculture production leads to increasing attention on the management of ammonia from its waste. Natural solutions such as Yucca schidigera extracts have shown beneficial effects on shrimps [1]. However, Y. schidigera is collected from the wild and its slow development make it an endangered natural resource that tends to be an expensive solution [2]. Alternative solutions, economically and environmentally improved, appear to be necessary to manage ammoniac. The aim of this study was to evaluate the effect of a dietary supplementation of a mixture of saponin-rich plants consisting of four saponin plants (Norponin Opti®, Nor-Feed) on the ammonia-nitrogen excretion of shrimps (Penaeus vannamei).

This study was performed in an experimental station (Halieutica, France). 138 juvenile shrimps were randomly divided into 2 groups with 3 replicates each: control groups (CTL), fed with a standard diet and supplemented groups (OPTI) fed with the standard diet supplemented with 500 g/T of the commercial blend of saponin-rich plants during 30 days. Measurement of TAN (NH4+/NH3) concentration in each group occurred once a day, 5 days a week using a colorimetric method assisted by a spectrophotometer. Individual performance measurements were made at the beginning and at the end of the experiment.

Results evidenced that OPTI group has a beneficial effect in reducing ammonia-nitrogen excretion during the whole experiment with an average difference of -13% and significantly (p<0,05) at week 3 of the experimentation with a difference of -20%. Weight gain during the experiment was not different significantly (CTL: 0.73 ± 0.15 g vs OPTI: 0.69 ± 0.15 g). Overall, the present study demonstrates that a supplementation with 500 ppm of the saponin-rich feed additive as positively impacting ammonia-nitrogen management.

References:

[1] Santacruz-Reyes RA, Chien Y-H. Bioresource Technology 2012; 113: 311–314.

[2] Arce-Montoya M, Hernández-González JA, Rodríguez-Álvarez M, et al. Plant Cell Tiss Organ Cult 2007; 88: 35–40.