Aquaculture America 2023

February 23 - 26, 2023

New Orleans, Louisiana USA

PHYTASE CAN REDUCE THE NEED FOR PHOSPHORUS SUPPLEMENTATION IN RAINBOW TROUT Oncorhynchus mykiss FEEDS

T. Gibson Gaylord*, Thomas Welker, Madison Powell, and Steven D. Rawles

 

Bozeman Fish Technology Center

US Fish and Wildlife Service

4050 Bridger Canyon Rd.

Bozeman, MT 59718

Gibson_Gaylord@fws.gov

 

 



Phosphorus is a required nutrient for rainbow trout and is supplemented as a mineral phosphate to meet fish’s dietary needs.  As plant-derived ingredients are increasingly utilized in trout feeds, increased P supplementation is often needed.  This is due to the inability of trout to digest phytate bound P commonly found in plants.  Increasing the efficiency of digestion and retention of P from the plant-based feed for rainbow trout will have both economic and environmental benefits for producers.  Phytase has been shown to be effective in releasing phytate-P in aquafeeds and in other animal industries supra-dosing phytase has shown benefits other than improving P digestibility.

To test the efficacy of phytase to release phytate-P in trout feeds to meet fish requirements and reduce P discharge, a 2 x 3 factorial experiment was conducted with rainbow trout to test phytase (Quantum Blue, AB Vista) at 0, 2500 and 7500 FTU/kg in a fishmeal-based and plant-based feed.  These formulations were also compared to positive control formulations with monocalcium phosphate supplying dietary P requirements.  Fish were reared in 15 °C in triplicate tanks of 40 fish weighing on average 31.9 g each for 12 weeks.  Feeds were extruded, floating 4mm pellets with phytase top-coated prior to final oil top-coating.

Rainbow trout grew less when no P was supplemented as monocalcium phosphate and no phytase was added.  Interactive effects were observed for growth, feed conversion ratios and feed intake with phytase addition between fishmeal-based and plant-based diets.  In plant-based feeds, final fish weight increased form 153 g when no P or phytase was supplemented to 208 g with supplemental P and an average of 213g when phytase was utilized.  Phytase improved FCR from 1.14 to 0.90 in plant-based feeds with no effect on fishmeal-based feeds.  Phytase addition reduced water-soluble P waste loads by 43% from the fishmeal-based feeds and 56% from the plant-based feeds.