Global human population is predicted to approach 10 billion by 2050, prompting an increased need to produce protein to support human dietary demands. Aquaculture food production will help address this increased demand for protein production. Seafood farming has established roots in many Asian and island countries and is becoming a more common form of food production in the United States. The expansion of aquaculture requires social license vetting due to the importance of sustainability to the average U.S. consumer. Integrated multi-trophic aquaculture (IMTA) represents a potentially more sustainable approach to aquaculture than traditional monoculture of a species within a single trophic level. IMTA is a method to produce multiple crops for market across a multitude of trophic levels. The most common IMTA arrangement is carnivorous finfish production coupled with production of secondary products for market, such as algae or bivalves, using the nutrient rich effluent water from the finfish culturing system to grow the extractive species. Research is being carried out at the University of Miami examining the aquaculture potential of four different macroalgae species (Agardhiella subulata, Caulerpa racemosa, Eucheum isiforme, and Ulva lactuca) in a pilot-scale IMTA system coupled with yellowtail snapper (Ocyurus chrysurus) production. Water quality sampling is being carried out over the course of the research trials, while macroalgae (“seaweed”) samples are collected every 3 days. The seaweed samples are assessed for compositional (protein, lipid, ash, and carbohydrate) analysis at the beginning and end of the research trials. Additionally, the carbon and nitrogen isotopes of the seaweed are being analyzed from the samples over the course of the project. Results of this work will provide detailed information on the bioextractive capacity of the selected macroalgae species when cultured in IMTA applications. The seaweeds are also being assessed from a market potential standpoint through collaborative work with local chefs and seafood distributors to assess the estimated market value of each species. The results of this project will provide novel information for existing and prospective IMTA operations throughout the Southeast U.S. and Caribbean regions and will allow producers to make well-informed decisions on candidate culture species of macroalgae for IMTA systems. This work has been supported by the Gulf States Marine Fisheries Commission (GSMFC) and the National Oceanic and Atmospheric Administration (NOAA).