Arkansas is home to the baitfish, sportfish fingerlings, hybrid striped bass fingerlings, largemouth bass, triploid grass carp, and feeder goldfish. Arkansas baitfish and sportfish farms, and state fish hatcheries routinely combat Columnaris disease, caused by Flavobacterium covae. Columnaris outbreaks are especially prevalent during feed training and when holding fish in vats. Understanding the immunological responses of fish to columnaris, would be useful to understanding how fish respond to columnaris outbreaks. Sixty channel catfish were stocked into separate 190 L tanks, which were part of a recirculation system. Thirty fish were injected with lipopolysaccharide (LPS) and 30 with physiological saline (PBS). Five fish were sampled from each group at 6, 24, and 72 hours. Blood, spleen, gills and liver were sampled from each of the five fish. Cortisol and gene expression were examined. Data analysis is currently ongoing, and results will be analyzed and presented.