Mass culture of algae in round tank of 500 liter capacity with internal illumination tubes were used for the study. These tanks had 5 tubes each having a length of 120 cm. in each tube 200 light emitting diodes of white light were placed which had illumination control. Three 60 watt flood LED (red) lights were also placed on the roof of the tank. The resultant illumination was 170µmol/m-2.The illuminations for the tanks was fixed for 16 hours daily with low illumination during start and shut down time of a cycle. The tanks were filled with 0.5M Sea water and a marine micro-algae Dunaliella salina was used as a organism in this study. The tanks were fed with 10% CO2 and 90% N2 mixture of gas for a period of 30 minutes a day with a flow rate of 3 L/min, for every hour 1.25minutes was set for dosing with micro-bubbles through a ceramic diffuser. The tanks were fed with three different modified D. salina media, Nitrate based media, Ammonia based media and fish paste based media for making highest biomass in period of 10 days. The highest biomass was recorded in fish paste based media with a 18.76x106 cells/ml, a count of 15.2x 106 cells/ml in nitrate based media and 14.89x106 cells/ml in ammonia media. The total wet biomass generated were in the range of 1.97g/L, 1.6g/L, 1.56g/L for the three media respectively.