Clownfish (Amphiprion ocellaris) are the most recognizable and popular marine aquarium fish and are a key commodity for marine ornamental fish producers. Although these fish have been raised in captivity for decades, producers are still experiencing issues regarding larval survival, reliance on live feeds, and post-larval deformities. To improve the culture process and reduce reliance on live feeds, the ontogeny of larval development and digestive capacity, namely stomach development, was examined. The timing of stomach functionality indicates when larvae have the capacity to better digest and utilize inert microdiets (MDs) that can be used to replace costly live feeds during larval rearing. The morphological development, digestive enzyme ontogeny, and digestive tract development of A. ocellaris was examined in a 16-day trial. Standard microplate assays were conducted to quantify the activity of trypsin, lipase, and acid protease enzymes from 24 hours before hatching to 15 days post hatch (DPH). Histology was used to visualize morphological changes in the digestive tract throughout the larval period. At hatching, A. ocellaris has functional eyes, an open mouth, and a coiled, differentiated digestive tract. Gastric gland formation was observed at 6 DPH, with stomach functionality occurring between 7 – 9 DPH, indicated by gastric gland proliferation and an increase in acid protease activity (Fig. 1-A). Lipase (Fig. 1-B) and trypsin (Fig. 1-C) activities were detectable before hatching and increased significantly after 6 DPH. The timing of stomach development from this study will be used to design a weaning trial to transition larvae from live feeds to MD without affecting larval growth or survival. Overall, understanding larval digestive ontogeny can aid in streamlining larval rearing and reducing reliance on costly live feeds.