Dietary protein is the most expensive component of the feed and an important determinant in L. vannamei growth. Therefore, understanding the interaction between dietary protein levels and daily protein intake which drives the shrimp’s growth is crucial. A 70-day feeding trial was conducted to illustrate the effect of dietary protein levels on the growth response of Litopenaeus vannamei reared in individual biofloc system each containing 800L of culture water stocked at 150 shrimp/m3. Four diets with crude protein levels of 25%, 30%, 35%, and 40% fed at the standard feeding rate were used for this experiment as four treatments. 30% CP and 35% CP diets were also fed at 133.3% and 114.3% of the standard feeding rate (equivalent to 40% CP), respectively as two other treatments. This resulted in a total of six treatments with four replicates each per treatment. At the end of trial, significant differences (P<0.05) in growth and FCR were observed. Results showed that the increase in crude protein content of the feed resulted in the increased final mean weight and weight gain. The shrimps fed with 35% CP diet at 114.3% had the highest final mean weight (14.1 ± 0.93g), weight gain (13.87 ± 0.94), and weight gain percentage (4490.73%). This trial demonstrated that the shrimps raised in intensive biofloc system perform better in terms of growth and feed utilization when provided with higher levels of crude protein diet.