This study was conducted with the target to establish a cryogenic sperm bank of Catla, Bighead carp and Grass carp for production of quality seeds using cryopreserved sperm in commercial hatcheries which becomes a prime need in this sector. Quality broodstock of Catla was developed by rearing Halda and Padma river-origin fish. Similarly, broodstocks of Bighead carp and Grass carp were developed by rearing recently imported China-origin fingerlings of these species. To standardize the sperm cryopreservation protocols of these species, activation of sperm motility was evaluated in different concentrations of NaCl solution (0.1 to 1.0%). Highest motility and swimming duration of sperm of Catla (95.3±1.5% and 37.6±0.6 min), Bighead carp (96.0±0.6% and 20.6±0.6 min) and Grass carp (96.3±0.3% and 21.9±0.7 min) were observed at 0.4 % NaCl solution. The toxicity of cryoprotectants (DMSO and methanol) to sperm was tested at 5, 10 and 15% concentrations for 5–40 min incubation with two extenders, Alsever’s solution and egg-yolk citrate, and better motility was observed at 5% and 10% concentrations during 5 and 10 min exposure. Alsever’s solution with 10% DMSO at 1:9 dilution (sperm : diluent) provided best equilibration (Catla 93.3±1.7%, Bighead carp 95.3±1.2% and Grass carp 93.3±1.7%) and post-thaw (Catla 86.7±1.7%, Bighead carp 89.7±1.5% and Grass carp 85.0±2.9%) motility of 10-15 min equilibrated sperm in cryogenic freezing. Breeding was conducted in eight government and private hatcheries for Catla, six for Bighead and five for Grass carp using cryopreserved sperm in different geographic locations and seeds were produced. The average fertilization and hatching were obtained as 38.1±3.2% and 31.0±3.1% in Catla, 41.4±2.0% and 31.8±1.3% in Bighead carp and 28.2±5.8% and 21.4±5.3% in Grass carp with cryopreserved sperm, whereas 77.3±4.2% and 65.2±4.3% in Catla, 76.3±5.4% and 60.9±6.3% in Bighead carp and 69.1±7.6% and 59.4±7.2% in Grass carp with hatchery-origin fresh sperm, respectively. Seeds of both cryopreserved and fresh sperm-origin are stocked in separate nursery ponds in respective hatcheries and being reared with supplementary feeds for comparing growth performances as well as brood production.