Asian seabass is one of the most cultured aquaculture species particularly in Southeast Asian region due to its remarkable tolerance for a wide range of environmental fluctuation. Salinity is one of the water quality indicators that has a direct impact on the metabolism and osmoregulation of fish. Therefore, the effect of different salinities (0, 15, and 30 ppt) on growth performance, body indices and feed efficiency of Asian seabass, Lates calcarifer were examined in this study. The experimental fish with an initial body weight (BW) 254.50 ± 21.70 g and total length (TL) 26.18 ± 0.66 cm were used, and groups of 10 fish per tank in triplicate group were distributed in 700 litre tank with recirculation and filtration system. The experiment was conducted for 85 days. Survival, growth in BW, body weight gain (BWG), total length (TL), total length gain (TLG), specific growth rate (SGR), condition factor (CF), hepatosomatic index (HSI), viscerosomatic index (VSI), intraperitoneal fat (IPF), total feed intake (TFI), daily feed intake (DFI) and feed conversion ratio (FCR) were evaluated. Asian seabass reared in 15 ppt attained significantly higher BW (470.40 ± 41.16 g), TL (31.51 ± 0.81 cm) and BWG (180.57 ± 24.85 g) compared to 30 ppt. However, there are no significant difference in survival, TLG, SGR, CF, HSI, VSI, IPF, TFI, DFI and FCR. The findings in this study showed that 15 ppt is able to promote better growth performance in Asian seabass compared to other salinities.