Two technologically advanced high protein distiller feed products, ANDVantageTM 40Y and ANDVantage 50Y (HP50Y & HP40Y) were evaluated for juvenile tilapia. This experiment assessing the nutritional potential of using high protein (40 and 50%) distillers dried grain products to replace corn protein concentrate (CPC) in formulation of tilapia diets. A 10-week growth study was conducted, using nine diets formulated to contain 32% protein and 6% lipid. Each protein was included at levels (0, 5, 10, 15 and 20%) replacing CPC on protein basis, with the 0% diet serving as a common control. Juvenile tilapia (mean initial weight 5.23 ±0.20g) were evenly distributed in thirty-six, 75-L aquaria working as a recirculating system and fed twice daily to apparent satiation throughout the study. Tilapia exhibited no significant (p>0.05) differences in growth, FCR, survival, whole-body proximate composition, mineral composition, and hematological parameters when fed HP50Y and HP40Y supplemented diets compared to the control diet. Results from this study revealed that HP50Y and HP40Y both are good alternative protein sources and can be used up to 20% inclusion level in the diets of tilapia.