Disease control is one of the most critical issues in sustainable aquaculture. In the past, antibiotics were used for bacterial disease as an effective treatment. However, as the Blue transformation is the future trend, antimicrobial resistance as an issue has been raised. Therefore, scientists seek a sustainable and eco-friendly method to control aquaculture diseases.
In this study, mangrove plant-based extract was tested for its anti-vibrio efficacy. The product has been tested for the presence of antibiotic residues and heavy metals. Subsequently, the product anti-vibrio activity was assessed in in vitro for minimum inhibition concentration (MIC). In vivo protective efficacy was studied using LD50 dose of vibrio challenge strain on shrimp. Immersion treatment of the experimental shrimps was performed, and a PBS injection group was included as a negative control.
All the experimental shrimp groups were monitored for up to 7 days post vibrio challenge. Prophylactic treatment of the shrimps demonstrated a significant protection against vibrio infection found with up to 50% reduction in mortality rate compared to the no treatment groups.