Brown adipose tissue (BAT) is known to consume ATP via mitochondria and thermogenesis. There have been many studies on differentiating BAT through Cold exposure and chemical treatment, However, studies on fish are unclear. Here, we present how to induce BAT in zebrafish without genetic modification. F irst, in brown preadipocyte t37i, a drug that induces BAT differentiation was treated, changes in gene and protein levels were confirmed. Second, at the animal level after exposing zebrafish to a high-fat diet and cold, check the differentiation of white adipose tissue (WAT) and BAT generation. This was reconfirmed by changes in gene and protein expression.
This study can be used as a basis for the study of brown fat in fish and provides inspiration for the use of zebrafish as a brown fat model species.