The bacterial community characterization of exsitu biofloc was carried out to ascertain the dynamics and community structure of the microbiome. The biofloc was developed in 50tonne raceways for a period of 30 days. Distillery spent wash as carbon source was used for maintaining the C: N ratio at 10:1. The harvested biofloc powder was analysed for the mineral profile using MS analysis. Metagenomic profile of the floc was done through shotgun sequencing using Illumina NextSeq500 platform. High quality data of 3.31 GB was obtained consisting 489,136 genes. The kingdom level abundance was found in the order of 62.47% of bacteria, 36.77% of unclassified, 0.4% eukarya, 0.32% of archaea, and 0.04% of viruses. At the generic level composition, Proteobacteria and Leptolyngbya sp. were the most abundant genus identified. Gene ontology using KEGG and SEED classification revealed predominantly carbohydrate metabolism associated genes. The present study is first of its kind to study the microbial composition in exsitu biofloc and to identify the functional attributes of the microbiome in the biofloc system.