Acadian Marine Plant Extract Powder (AMPEP) is an organic fertilizer derived from extracts of brown algae (Ascophyllum nodosum) that is commonly used to increase the productivity of agricultural crops. However, it has the potential to be used as a cheap and sustainable microalgae culture medium for the production of lipid and β-carotene. This study aims to determine the effect of variations in the concentration of AMPEP on the growth, lipid and β-carotene content of Dunaliella salina, a well-known microalgae species using in food industries. This study used 4 AMPEP concentration (0.01, 0.1, 1, 10) ppm and Walne in triplicates. The initial density of the culture was 105 cells.mL-1 cultured for 7 days in plastic bottles (v=600 mL) with a culture volume of 300 mL. Microalgae density was calculated every other days using an improved Neubaur haemocytometer and a hand counter under a microscope with 40x magnification. Microalgae were cultured at light intensity 16.2 moles.m2.s-1 , temperature 28°C, pH 7-8 and salinity 29-30 ppt. Microalgae were harvested using pre-dried, pre-weighed Whatman microfiber filter paper (O=25 mm), dried (1 hour, 100°C) and ashed (5 hours, 450°C) then weighed to calculate dry weight and ash-free dry weight. Density data during the culture period were analyzed using Repeated Measure ANOVA while data on specific growth rate, dry weight (DW), ash free dry weight (AFDW), biomass productivity and lipid content used One-Way ANOVA. Lipid analysis was performed using modified Bligh and Dyer method. The content of β-carotene was analyzed using spectrophotometer UV-VIS at absorbance of 450 and 665 nm. The interaction between day and concentration of AMPEP media affected the growth of D. salina (p=0,000). The final density, relative growth rate and the highest biomass productivity were found at the AMPEP media concentration of 10 ppm (p=0,000) with values 418,8×104 cells.mL-1 , 1.003 cells.day-1 and 1.35 g.L.day-1, respectively. The highest lipid content was found at a concentration of 0.01 ppm that was 5.26%. The highest β-carotene content was obtained at a concentration of 10 ppm AMPEP medium of 0.3545 ug.mL-1 (p=0,000), whereas in concentrations of 0.01, 0.1, 1 ppm and Walne, the β-carotene content did not differ, ranging from 0.1-0.2 g.mL-1 . This study showed that the concentration of AMPEP media of 10 ppm may be used for Dunaliella salina culture for the production of β-carotene as a natural antioxidant although the lipid content was considerably low.