An optimal broodstock diet contains essential nutrients that are required for successful reproduction and healthy offspring production. Currently, a cut-bait diet is commonly used by the industry to feed broodstock. However, a formulated pellet commercial diet would be a more affordable, sustainable and nutritionally consistent alternative. The aim of this study is to use alternating diets of sardine-squid and commercially available pelleted feed to determine the amount of time needed for broodstock to incorporate nutrients, especially essential lipids, from their diet into their eggs. California yellowtail, Seriola dorsalis, broodstock were fed two alternating diets: Vitalis Prima pellets (World Feeds Ltd, UK) and sardine-squid. Each diet was switched after every 6 weeks within the spawning season lasting for a total of 24 weeks and 47 spawns. Egg samples along with biometric data from each spawn were collected. They will then be processed for proximate analysis, essential fatty acid content, and amino acid composition. This data will be used to compare the varying nutritional incorporation happening at each diet switch. This study aims to provide a greater understanding and insight into nutritional assimilation on a temporal scale between the maternal parent and offspring. Our findings will contribute towards the development of a more cost-effective and reliable broodstock diet for farmers and reduce the industry’s reliance on cut bait diet, which will improve the economic viability of the California Yellowtail as a staple aquaculture species.