In shrimp farming viral diseases produce important economic losses. The use of prophylactic methods as natural additives wthout inmmune resistance and environmental problems were proben. Medicinal plants powder, yeast and B. licheniformis were used in the survival in the digestive and immune systems of P. vannamei challenged against V. parahaemolyticus.
Four bioassays were carried.Postlarval white sThe combination of MP in the food and B. licheniformis in the water (3 x 106 CFU / L) significantly improved the survival of P. vannamei challenged with V. parahaemolyticus. B. licheniformis inoculated in the water, did not alter the expression of the trypsin digestive gene.
B. licheniformis in the water decreased the expression of the SOD gene (related to the immune system), which plays an important role as an antioxidant, decreasing the concentration of superoxide anion, the product of the phagocytosis process. B. licheniformis in the water did not alter the expression of the genes of the immune system penaeidine4 and lysozyme. The mixture of MP added in the feed and B. licheniformis in the water prevent the AHPND in P. vannamei cultivated in the laboratory.