Rotifers are small zooplankton that live in freshwater, brackish and marine environments. They are used extensively in aquaculture because of their high reproduction rate, optimal size for larval fish and good nutritional profile. Rotifers are essential for the first feeding of many species of marine finfish larvae. The commonly used rotifer culture methods by hatcheries in Singapore and the region are indoor batch cultures and outdoor pond cultures. However, the two methods could face culture stability issues where the rotifer population collapse overnight, and the shortage of rotifers resulting in the failure of the affected larviculture batch. In addition, an outdoor pond culture has a larger footprint compared to indoor batch culture. This topic describes the rotifer culture method based on a recirculating aquaculture system (RAS) developed at the Marine Aquaculture Centre and the management protocols adopted to maintain stability of rotifer growth. In a land-scarce Singapore, having this technology would enable production of rotifers intensively and consistently on a small footprint, which is important to support large-scale hatchery production.