ANTIMICROBIAL POTENTIAL OF SOLID STATE FERMENTED AGROINDUSTRIAL BY-PRODUCTS' EXTRACTS IN MARINE FISH
Silva, J.F.1*, Serra, C.R.2, Santos, R.A.1,2, Oliva-Teles, A.1,2, Fernandes, H.1,2 , Diogo, F.1, Belo, I.3, Salgado, J. 3, Castro, C. 2, Peres, H.1,2
*joaomanuelfelixsilva@gmail.com
1FCUP-Biology Department, Faculty of Sciences of University of Oporto
2CIIMAR-Interdisciplinary Centre of Marine and Environmental Research, University of Oporto
3CEB-Centre of Biological Engineering of University of Minho
In order to ensure the future global fish provision, sustainable development of aquaculture is mandatory. To guarantee the total quality and consumer safety of fish provided by aquaculture, alternative nutritional strategies for bacterial disease management are required, preventing the use of synthetic drugs.
Agroindustry byproducts, like those from winery and olive oil industry constitute a low cost source of add-value bioactive compounds. Solid-state fermentation(SSF) is a biotechnological process that may be applied to increase the availability of their bioactive compounds.
The aim of this study was to develop an innovative bioactive enriched extract from the SSF of solid agroindustry by-products with Aspergillus ibericus and assay their antimicrobial potential against fish pathogens.
For that purpose, optimized mixture to maximize antioxidant potential of exhausted grape marc, vine trimming shoots and exhausted grape marc, were fermented by A. ibericus MUM 03.49. Fermented and unfermented matrixes were water extracted and their total phenolic content was determined. The antimicrobial potential of these extracts against high incidence fish pathogens in aquaculture was addressed by spot analysis and well-diffusion assays.
The unfermented optimized mixture extract has shown to be effective against bacteria from Aeromonas, Edwardsiella, Photobacterium, Vibrio, Staphylococcus and Streptococcus genera, while fermented optimized mixture inhibits Streptococcus iniae and Photobacterium damselae subsp. piscicida.
Further analysis are being conducted to address potential fermented and unfermented optimized mixture extracts against bacteria from Tenacibaculum genera.
Funded by:Project SPO3(ref.POCI-01-0145-FEDER-030377;FCT); project InovFeed(ref. MAR-02.01.01-FEAMP-0111;Mar2020).