The aim of the study is to determine the optimal stocking density in rearing larvae of white shrimp (Litopenaeus vannamei) under biofloc condition. The experiment consisted of four triplicate treatments with different stocking densities of (i) 150 larvae/L, (ii) 200 larvae/L, (iii) 250 larvae/L and (iv) 300 larvae/L. Larvae were stocked in 500-L tanks at water salinity of 30‰ with continuous aeration. Sugar was used as carbon source and added every day to rearing tanks from the larval stage of Mysis-3, with ratio of C:N of 20:1. Results showed that water quality parameters, total bacteria, Vibrio spp bacteria count and floc volume were in suitable range for larvae development. Postlarvae-12 length and survival rate were the highest at stocking density of 150 larvae/L (11.59 ± 0.16 mm; 58.7 ± 7.9%), and it was not significantly different compared to those at stocking density of 200 larvae/L (11.49 ± 0.38 mm; 55.0 ± 3.0%) (p>0.05). The highest yield was found at stocking density of 200 larvae/L and it was significantly different to remaining treatments (110±6 post-larvae/L) (p<0.05). In short, rearing white-leg shrimp larvae at stocking density of 200 larvae/L under biofloc condition presented the best practice.