RNA interference (RNAi) mediated gene silencing of pathogen genes has been demonstrated to be an effective means to control viral replication. The challenge, however, has been how to continuously produce and deliver RNAi molecules in host animals for pathogen control. Pebble Labs has developed a platform technology called Directed Biotics that continuously produces and delivers RNAi molecules to the host using engineered symbiotic bacteria naturally associated with the host. Previously, we have demonstrated that feeding shrimp a generic RNase III-deficient Bacillus subtilis strain expressing dsRNA targeting essential white spot syndrome virus (WSSV) genes could substantially reduce shrimp mortality. Improved Bacillus strains were then selected which had the greatest efficacy for reducing pathogen numbers using RNAi. These Bacillus strains were then engineered to express a dsRNA targeting the silencing of an essential WSSV gene, Vp19.To test the efficacy of the Directed Biotics WSSV solution, shrimp were fed Bacillus expressing dsRNA-Vp19 or commercial food without bacteria for 5 days prior to a White Spot Syndrome Virus (WSSV) challenge by injection. Full protection (zero mortality) was observed at 10 days post WSSV infection for shrimp fed food pellets top-coated with Bacillus expressing dsRNA-Vp19 for 5 days prior to WSSV challenge. The negative control group had 85% mortality. Additionally, qPCR analysis of WSSV titer indicated there was a 3-log fold reduction in virus titer in surviving shrimp fed Bacillus expressing dsRNA-vp19 compared to the control group. In summary, we demonstrated that the Directed Biotics platform technology can provide full protection against WSSV challenge in the lab. Similarly, we have applied the Directed Biotics technology to control EMS (Vibrio) bacterial infections in shrimp. In contrast to viral control strategies, we engineered our Directed Biotics bacteria to express anti-sense RNA (asRNA) to target suppression of the Vibrio encoded DNA adenosine methyltransferase (dam) gene to reduce Vibrio DNA replication and toxin production. Following a Vibrio challenge of shrimp pre-feed our asRNA expressing bacteria, we observed an 80% reduction in toxin production and 50% survivability of shrimp challenged with a lethal dose of Vibrio. Similar applications of Directed Biotics technology for the control of viral diseases in plants has resulted in 100% suppression of viral replication demonstrating the broad efficacy of the Directed Biotics platform technology. Collectively, Directed Biotics represents a novel, cost-free means to produce and deliver targeted RNA molecules in hosts to inactivate pathogen genes with no-off target effects; potentially eliminating the need for antibiotics in the field.