Management of ice ice free seaweed aquaculture is important in increasing seaweed production recently. For that reason, it is required a technology that is able to support the health of seaweed. This research aimed to determine the optimation, specifity, and sensitivity of specific primer and to develop molecularly quick detection method to detect the agent of ice ice disease on seaweed thallus. Optimation, specifity and sensitivity tests as well as detection on thallus used specific primer PCR (aSEFM-F and aSEFM-R). PCR was conducted as follows: pre-denaturation at 94°C for 5 minutes, synthesis at 72°C for 2 minutes, post PCR at 72°C for 7 minutes, and PCR reaction was stopped at 4°C. Research result showed the use of PCR with specific primer aSEFM-F and aSEFM-R was able to detect isolate Vibrio alginolyticus PNGK 1 from pure culture and to detect ice-ice disease directly on seaweed tissue within 6 hours with DNA concentration of 0.21 ng/µL, while at concentration bacteria cell at 2.3 x 103 cell/mL and high specificity indicated by the present of band electrophoresis get at 201 bp with comparative evaluation of bacteria Vibrio alginolyticus SKT-b, Vibrio harveyi, Pseudomonas cepacia, Flavobacterium meningosepticum, Pseudomonas diminuta and Plesiomonas shigelloides.
Keywords: Vibrio alginolyticus PNGK 1, aSEFM-F and aSEFM-R, optimation, specificity, sensitivicity