The Cyperus rotundus rhizoma is reported to have methyl fernesoate compounds which are identical to Juvenil hormone III, Juvenil hormone in crustase (methyl fernesoate) can stimulate the activity of other hormones such as ecdystetron and serotonin which play a role in the regulation of metamorposis and maturation of the male and female gonads. The objectives of the study include the isolation of the methyl fernesoate compound from cyperus rotundus rhizome, then the application of the cyperus rontundus extract to the broodstock of tiger shrimp through injection at a dose of 0.100,150, and 200 ug / g bb, and the ablation of the eye stalk as a positive control. The study was conducted using a completely randomized design (CRD) method with 5 treatments and 4 replications. To determine the frequency of molting, gonadal maturation level, number and diameter of eggs and measurement of the rate of development of vitelogenesis by PCR technique using primary vitellogenin F, R and β-Actin F, R. The observations showed that the highest molting frequency was at 150 ug / g bb treatment that was 15 times during maintenance, and the parent shrimp that had the highest gonad maturity was at 150 ug / g bb treatment as much as 75%. The results of the measurement of the rate of development of vitelogenesis through a molecular approach with PCR technique using the vitellogenin primers F, R and β-Actin F, R showed a positive development in the broodstock shrimp treated with Cyperus rontundus extract
Methyl fernesoate (JH III) plays a role in stimulating and coordinating reproduction in insects (Wyatt, 1997), contributing to the reproductive system such as vitellogenin, controlling the immune system, and the resulting embryos (Gaubard Y, 2005; Min et al., 2004; Masner et al., 2004). al., 1968). In MF crustaceans act as gonadotropins and growth, and this hormone is found in the vitellogenin of female individuals and active reproduction in males (Laufer et.al, 1993 in Enrique, et.al., 2001). Methyl Fernesoate (JH III), besides being found in crustaceans and insects, is also found in several types of plants, including in the grass of grass (Cyperus iria) (Bede et al 2000), in cananga flowers (Cananga latifolia) (Yang et 2013) and several types pine plant. So based on this information, the isolation of juvenile hormone (Methyl fernesoate) from cyperus rontundus can be used as a molting stimulant and the development of reproductive organs in tiger shrimp.