Aaptos aaptos was well known to contain aaptaminoid used as natural antibacterial source without side effect. This research aims to get bioactive compounds of Aaptos aaptos butanol extract which were able to inhibit the growth of Vibrio harveyi and to evaluate the efficacy of the compound in prevention of V.harveyi infection
Metabolite secondary extraction used maseration method, while isolation and identification of aaptaminoid used column chromatography and spectroscopy method as well as comparison to the published data. The antibacterial test against V.harveyi used agar difussion method with paper disc. Evaluation of the active compound in Vibriosis prevention used experimental method with 4 treatments. Each treatment used L.vannamei of 10 ind./aquarium. Dosages of the active compound used namely; A. 0 µg/g bb, B.0,67 µg/g bb, C. 25 µg/g bb, and D. 50 µg/g bb. Injection of 100 µL of bioactive compound at each dosage was carried out at the initial experiment and at 14 days of rearing time the tested shrimps were challenged with V. harveyi at the density of 107 CFU/mL The rearing of shrimps was continued for 7 days. Finding showed that the infection on shrimps started on 23 hours post injection of V,harveyi indicated by reddish colour of rostrum, body, pereopoda, pleopoda, and telson. The shrimps on the treatment C and D were recovered from the infection started on the day-4 post infection exhibited by the normal rostrum, body, pereopoda, pleopoda, and telson. The highest survival rate post infection was obtained on treatment D (50%) and that of treatment C was 25 %. In turn, shrimps on treatment B and A did not show any survival rate. It indicated that aaptaminoid at the dosage >25 µg/g bb can be developed as an antibacterial agent for Vibriosis prevention.