The effects of first feeding on the survival, growth and behaviour of larvae Amur catfish, Silurus asotus in tropical region were examined under controlled conditions. The total length, yolk sac volume, yolk sac absorption time, nutritional transition period (NTP) and behaviour of the larvae were observed. Larval first feeding time was indicated when the eyes were pigmented, the mouth had opened with a functional lower jaw, the intestine had peristaltic movement and the anus was opened. Larval first feed intake was observed at 30 hour after hatching (hAH) with total length 7.26 ± 0.24 mm at 27.2 ± 1.0 ?C. Larval first fed at 30 hAH with yolk sac volume, 0.33 ± 0.08 mm3 and the yolk sac absorption completely at 60 hAH (yolk sac volume, 0.10 ± 0.01 mm3) with larval total length, 8.32 ± 0.51 mm. Larvae was feed with combination of live feed, rotifer (Brachionus plicatilis) and formulated pellet (Otohime, Nisshin Maru, Japan). The NTP of the Amur catfish larvae was 30 hours. The survival of Amur catfish larvae was 98.0 ± 0.6% at first feeding (30 hAH) and decreased to 90.0 ± 0.7% (60 hAH). At first feeding, larvae continuously moved at the bottom of the tank and sometimes swam vertically and larvae have active swimming at this stage. Hence, larvae swam mostly at the bottom of the tank and sometimes in the middle and surface layers at 60 hAH.