The selection of broodstocks resistant to infectious diseases is a proven sustainable and accessible measurement to improve fish health. In this study, lumpfish that survived the intraperitoneal challenge with Aeromonas salmonicida or Vibrio anguillarum was selected. These fish were quarantined and grown until they reached sexual maturity. Resistant males were crossed with resistant females and domestic females. Similarly, resistant females were crossed with domestic males. A few individuals of the F1 generation from the resistant cross showed an eye deformity. After the F1 generation reached an average weight of 73g, they were bath challenged with V. anguillarum (1 x 104 CFU/mL) for 30 minutes. At 42 days post-challenge, the relative percentage survival (RPS) of the resistant cross was 43.05%. The RPS for the resistant female crossed with the domestic male and the resistant male crossed with the domestic female was 6.77 % and 0%, respectively. These results suggest that the lumpfish resistance to V. anguillarum could be conferred by a recessive set of genes.