Background
An ideal model for studying Vibrio and host and their interaction in disease dynamics
is Crassostrea Gigas in the North Sea, exposing invasive sources to pathogens,
especially (vibrio kanaloae).
Methods
This study used molecular fluorescent in situ hybridization (FISH) techniques to
rapidly identify the diversity of bacteria in the oyster Crassostrea Gigas from Sylt
Island and 16SrRNA gene sequence validated all strains’ identification. Oysters were
collected and exposed with vibrio kanaloae strains on different tissues were
examined utilizing culture-independent methodologies.
Results
the digestive glands, gill and Muscle in vibrio Kanaloa were identified as
metabolically active by the FISH technique, one of the advantages of the FISH
technique is that it is accurate and very easy to use.
Conclusions
This technique allows rapid assessment of Vibrio in oysters and seafood.
Keyword 1
Vibrio kanaloae
Keyword 2
oyster Crassostrea Gigas
Keyword 3
Fluorescent in situ hybridization