Of the Brycon moorei endemic species of Colombia, it is not known about the enzymatic activity and its modulation concerning diets formulated in the early stages. In a recirculating set-up and with a completely randomized quintuplicate design, an ad libitum feeding protocol was applied, which included, among others, balanced feed with inclusion levels from 35% to 55% of crude protein (CP). For this, larvae 22 hours post-hatching (HPH ), with an average initial weight and length of 3.07±0.69 mg and 6.069±0.22 mm, respectively, were seeded at a density of 17 larvae L-1 in tanks with an effective volume of 60 L. The enzymatic activity was analyzed about the experimental diets. Specific activity (SA) of serum enzymes (Figure 1, 2, and 3) was found from the beginning of hatching, with fluctuating values ??significantly related to the feeding protocol and time in HPH and the pepsin-type SA began from the 111 HPH, marking the time in which the use of dry diet can be started (Figure 4).