World Aquaculture 2021

May 24 - 27, 2022

Mérida, Mexico

IS THERE ONLY ONE SPECIES OF Hepatozoon INFECTING BRAZILIAN CAIMANS?  MOLECULAR ANALYSIS BRINGS NEW INSIGHTS INTO HAEMOGREGARINE DIVERSITY

Letícia Pereira Úngari*, Edward Charles Netherlands, André Luiz Quagliatto Santos, Edna Paulino de Alcantara, Reinaldo José da Silva and Lucia Helena O´Dwyer

 

Setor de Parasitologia, DBBVPZ, Instituto de Biociências, UNESP, Distrito de Rubião Junior

Botucatu (SP) Brasil.

letspungari@hotmail.com

 



The population of Brazilian caimans is considered the largest in the world. Brazil is the second-larges t exporter of fur and  Brazilian  alligator farming is a

 potential  aquaculture produc tion  in agribusiness

 and an alternative to reduce the disorderly and illegal ex traction of these wild animals.  Therefore, a strict sanitary scheme is necessary for

 the breeding sites to avoid the spread of diseases .  Among the infections that affect these animals, Brazilian caimans are hosts  of a great number of parasites, including hemoparasites .

 In 2018-2020 , eleve n Caiman crocodilus from Mato Grosso State were  collected and screened

 for haemogregarine parasites.  Through morphological  analysis,  a species of  the genus Hepatozoon (Adeleorina: Hepatozoidae) was identified  with free gamonts [Fig.1a], gamonts with cytoplasmic vacuoles [Fig.1b] and mature gamonts [Fig.1c] in the blood smears ,  and meronts with merozoites

[Fig.1d-f]

in the liver of these animals .

 The eleven isolates amplified targeting the 18S gene have shown 100% similarity among them, and 98.4% - 98.8% in comparison with other isolates of Hepatozoon caimani , the only species known  to infect  Brazilian caimans so far. Complementary to this, the  phylogeny revealed a main clade of isolates from Brazilian caimans,  divided into two subclades, one of this study and the other with Hepatozoon caimani isolates only [Fig. 2]. This result brings new insights on  Hepatozon  diversity infecting the caimans, with a possible new species reported in this study and generates important information for  diseases in alligator farming in Brazil.