Controlling oxidative stress in shrimps under culture conditions is of vital importance to have males with good physiological status. The use of diets supplemented with vitamins are widely used for this purpose. The physiological status of F. brasiliensis Latreille (1817) broodstock males fed with vitamin C supplemented diet s was determined. A formulated Diet was used as a control and three Diet s were prepared with three vitamin C levels: 894 mg/kg (Diet A), 1639 mg/kg (Diet B), 2384 mg/kg (Diet C). Triglycerides, cholesterol, glucose, and prophenoloxidase quantities; type of hemocytes; lipid peroxidation ; and total proteins in the hemolymph were determined. In addition, the antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (Gpx) were quantified. Differentially expressed genes of the immune system , namely those of a-2 macroglobulin (a2M), peneidin (Pen), hemocyanin (Hc), prophenol oxidase (ProFo) , and the genes encoding antioxidant enzymes SOD, CAT, and Gpx were evaluated. Sperm quantity and quality were recorded. In males fed Diet C , the lowest cholesterol values and triglycerides obtained in the hemolymph were not significantly different from those fed the control Diet (P < 0.05) . Shrimps fed Diet C showed the highest amount of hyaline hemocytes, the lowest CAT enzyme activity, and the highest Gpx activity. The immune system genes only showed differences between a 2M and Pen; sperm quantity and quality were not affected by diet . The results indicated that Diet C (2384 mg/kg) was effective for the maintenance of F. brasiliensis broodstock males because it controlled cholesterol and triglyceride levels; there was less CAT activity and less activation of the immu ne system mediated by hemocytes.