This study aimed to evaluate the effects of using a combination of commercial probiotic products as a feed supplement and as water additive on the water quality, growth, and survival on nursery culture performance of Penaeus vannamei. A four-week nursery trial was conducted using bacterial based water treatment. Two commercial probiotics were used as water additives singularly and in combination with a probiotic supplemented- feed, resulting in four treatments using static biofloc type systems. The fifth treatment was used as the reference by connecting to a recirculating aquaculture system (RAS). Results showed that the shrimp post larvae cultured in the four probiotic treatments had significantly higher final biomass (g) and survival (%) as well as significantly lower FCR than the reference (p < 0.05). There were no significant differences between dissolved oxygen (mg/L), temperature (ºC), pH, and total ammonia-nitrogen (mg/L) of the five treatments. However, a peak of total ammonia-nitrogen (1.11 ± 0.08 mg/L) and nitrite (0.74 ± 0.06 mg/L) was observed in the four probiotic treatments around day 20 of the experiment, that was rapidly decreased by day 22 (Figure 1). This is assumed to be due to the continuous addition of the fermented probiotic additive to the water as well as the restricted feed during these peaks. Overall, results of this study and other published studies indicated that biofloc type systems have a number of advantages over clear water systems and that the use of commercial probiotics helps to develop and maintain biofloc based systems for enhancing shrimp performance.