Aquaponics, an integration of aquaculture and hydroponic systems, is expected to become a surging technology to provide fish and vegetable production benefits while reducing problems associated with fresh water and reactive chemicals usage. However, information available on bioactive compounds especially phenolic profiles in leafy lettuce is lacking in aquaponics production systems . Both red and green lettuce samples were collected from replicated aquaponics and hydroponic systems (as a control ) and analyzed for their growth, yield, and antioxidant properties. All together nineteen phenolic compounds were analyzed in lettuce samples using LCMS technology. Results showed that aquaponics had a significantly higher (by 7%) leafy lettuce production when compared with hydroponics , coincided with a large accumulation of phenolic compounds. R ed varieties of lettuce had a high phenolics level than that of the green one . Among the phenolic compounds, the quercetin-malonyl glucoside , quercetin glucuronide, di-caffeoyl tartaric acid, ferulic acid were consistently higher in aquaponics lettuce when compared to the hydroponic lettuce. Out results suggested that the anti-oxidant quality of aquaponic lettuce is much better in context of public health claims when compared with hydroponic lettuce.