Aquaculture 2022

February 28 - March 4, 2022

San Diego, California

VERIFICATION OF RIVERS’S POSTULATES FOR BLUE CATFISH HERPESVIRUS

Vandana Dharan* and Suja Aarattuthodi

 

 Thad Cochran National Warmwater Aquaculture Center,

Mississippi State University, Stoneville, MS 38776

vd302@msstate.edu

 



Herpesviruses are a significant threat during the hatchery and nursery phases of catfish aquaculture.  Blue catfish alloherpesvirus (BCAHV) is a novel strain of ictalurid herpesvirus-1 isolated from blue catfish fingerlings.  The  genome of  BCAHV  and CCV shares significant similarities. A  detailed study on the pathogenesis of BCAHV revealed that it causes  substantial  mortalities in blue catfish (82%)  and  hybrid catfish (26%) fingerlings which can drastically affect the viability of farm operations.  Thomas Rivers (1937) modified  Koch’s  postulates to include viruses as infectious agents causing diseases . According to Rivers’s postulates, the causative relationship between a virus and disease are stated as isolation of virus from the diseased host, cultivation of virus in host cells, proof of filterability, production of comparable disease when the cultivated virus infects naive animals, reisolation of the same virus from the infected animals, and detection of specific immune response to the virus. Validation of theses six postulates are crucial to verify the causation of a disease as a virus. Fulfilment of Rivers ’s postulates is also essential to formulate  pathogen-targeted  treatment and management strategies including vaccination. Rivers’s postulates were tested and verified for BCAHV infection to confirm the causality of infection in blue catfish (Figure 1) . Specific immune responses against BCAHV were observed when the survivors from initial virus exposu re  were challenged with wild type virus. Hence, BCAHV fulfilled all  the criteria to be the primary etiological agent of infection in blue catfish.