Aquaculture 2022

February 28 - March 4, 2022

San Diego, California

EVALUATING FRESHWATER FLUIDIZED SAND BIOFILTER PERFORMANCE FOLLOWING EXPOSURE TO LOW-DOSE PERACETIC ACID TREATMENTS

Christine Lepine, Natalie Redman, Megan Murray, Carlo Lazado, Lill-Heidi Johansen, Åsa Maria Espmark, John Davidson, and Christopher Good *

The Conservation Fund Freshwater Institute

1098 Turner Road

Shepherdstown, WV 25443

cgood@conservationfund.org

 



Applying disease or pathogen control treatments in recirculating aquaculture systems (RAS) can be challenging due to the potential impacts of  the administered  chemical agent(s) on biofilter performance. Fish farmers utilizing RAS would benefit greatly from  possessing more  treatment options that are both efficacious  towards the intended  microbial  target(s) and relatively safe  in terms of maintaining biofilter function for sufficient nitrification of accumulating ammonia. One particular chemical, peracetic acid (PAA), has shown promise outside the United States for use in controlling disease and pathogen counts in commercial RAS; however, PAA’s use  in the U.S. aquaculture is currently restricted to  materials surface disinfection when food fish are not present. Before approval of PAA as a bona fide  aquacultural therapeutant is considered, baseline research is required to  assess, among other things, the impact on nitrification as RAS biofilters are exposed to PAA.

 To address this knowledge gap, we  exposed replicated experimental-scale  freshwater fluidized sand biofilters  to 4-hour exposures of  semi-continuous low-dose PAA at three separate concentrations, and monitored PAA concentration over time  to assess  peak concentrations achieved as well as chemical decay following cessation of treatments. Following PAA exposure, we monitored all biofilters for a period of two weeks and assessed total ammonia nitrogen (TAN) removal efficiencies, influent and effluent nitrite- and nitrate-nitrogen concentrations, and  bacterial biomass. At the time of abstract submission, the study is ongoing, with anticipated  final data collection in November 2021.  Final  PAA dose-response impacts on RAS biofilter performance, as well as recommendations for PAA use in RAS based on study results, will be presented at Aquaculture America 2022.