Microinjection is a widely used technique employed by biologists with applications in transgenesis, cryopreservation, mutagenesis, labeling/dye injection and in-vitro fertilization. However, microinjection is an extremely laborious manual procedure, which makes it a critical bottleneck in the field and thus ripe for automation. We have recently developed a computer vision-guided robotic platform that automated the targeted microinjection of zebrafish embryos , one of the most important model organisms in biological and drug discovery research. A computer vision guided microinjection robotic platform uses a series of cameras to image a Petri dish containing embryos at multiple magnifications and perspectives. This imaging is combined with a machine learning algorithm and computer vision algorithms to automatically detect 100s of embryos on a Petri dish and pinpoint a location on the embryo for targeted microinjection with microscale precision. Once located, the robot automatically guides each embryo on the Petri dish to the micropipette for microinjection. We demonstrate the utility of this microinjection robot to successfully microinject zebrafish embryos. Preliminary results indicate that the robotic microinjection has the potential to significantly increase the throughput as compared to manual microinjection. The performance of an automated microinjection robotic platform can be validated by microinjecting the cryoprotectants into the yolk of the zebrafish embryos for cryopreservation, which is a safe and non-toxic method . Survivability of zebrafish embryos in cryopreservation experiments is mainly affected by the variability of manual microinjection, physical damage due to microinjection and toxicity of the solution injected. The experiments to manually investigate these parameters to improve the survivability of zebrafish embryos will be time consuming and laborious. Therefore, we are using this robot to study the effects of microinjection on zebrafish embryos and seek to derive fundamental principles that can be generally applied in other contexts and species. We are also using this robot to develop novel cryopreservation and transgenesis strategies of zebrafish embryos. In the future, this robotic platform can be used to perform high throughput microinjection experiments and it can be extended to automatically microinject a host of organisms such as fruit fly (Drosophila melanogaster) embryos, roundworms (Caenorhabditis elegans), mosquito (Culicidae) embryos, sea urchins (Echinoidea), and frog (Xenopus) oocytes.