Fish aquaculture as an industry has developed significantly in order to offer human consumers with nutritious protein. Farmers, however, have been dealing with the impact of stress on fish growth, reproduction, and immunity as the industry has progressed. As a consequence, finding viable techniques to minimize stress in fish and enhance their capacity to resist disease, as well as lowering farmers’ reliance on chemical treatments, is imperative. In Southeast Asia, Gynura procumbens , also known as longevity spinach, is a popular medicinal plant that has been used to lower blood glucose levels, which is major concern in stressed animals. Our previous investigation (on acute stress) was to see how Gynura procumbens extract benefited stressed tilapia and to find an effective concentration for a chronic study. In this experiment, we are investigating the effects of 0.0015% Gynura procumbens on stress physiology and immunology of Nile tilapia for 12 weeks. In this experiment we are using four treatment groups including control group (w/o stress and GPE), control treatment group (w/o stress but w/ GPE), stress group (w/ stress but w/o GPE) and stressed treatment group (w/ stress and w/ GPE). Results from t his research will give us insight how effective Gynura extract is as stress mitigating substance. This research will be completed soon , and the findings will be presented at the World Aquaculture Society meeting.