Aquaculture America 2020

February 9 - 12, 2020

Honolulu, Hawaii

A REVIEW ON DIETARY PROBIOTICS AS ANTIBIOTIC REPLACES IN AQUACULTURE

Sungchul C. Bai, Ali hamidoghli, Seunghan Lee, Seonghun Won
Dept. of Marine Bio-materials and Aquaculture/Feeds & Foods Nutrition Research Center (FFNRC),Pukyong National University, Busan, 608-737, Rep. of Korea
 

Four feeding trials were conducted to evaluate the effects of dietary probiotics  as antibiotic replacers in Japanese eel Anguilla japonica , Nile tilapia Oreochromis niloticus ,  and whiteleg shrimp Litopenaeus vannamei . In the first experiment, growth performance, non-specific immune responses, immune-related gene expression and cumulative survival rate of Japanese eel fed Bacillus subtilis WB60 (0.5× 10 7 CFU/g )  with mannanoligosacharide  (5 g/kg) was significantly higher than those of fish fed control (CON) diet. However, there were no significant differences with the oxytetracycline (OTC) diet. In the second experiment , growth performances, non-specific immune responses, cumulative survival rate of Nile tilapia fed  B. subtilis at 1 × 108 (CFU/g ) and Lactococcus lactis at 1 × 108 (CFU/g)  diets were significantly higher than those of fish fed CON diet. Immune-related gene expression and enzyme activity  of these diets were significantly higher than those of CON and OTC diets. In the third experiment, growth performance , non-specific immune responses and cumulative survival rate of whiteleg shrimp fed  B. subtilis at 1 × 108 (CFU/g)  and L. lactis at 1 × 108 (CFU/g)  diets were significantly higher than those of shrimp fed  CON diet. Immune-related gene expression and histology of shrimp fed these diets were significantly improved compared to those of shrimp fed CON and OTC diets.  In the fourth experiment, g rowth performances, non-specific immune responses, immune-related gene expression and cumulative survival rate of Nile tilapia fed  B. subtilis at 1 × 108 (CFU/g) , Enterococcus faecium 1 × 107 (CFU/g), and E. faecium 1 × 108 (CFU/g) were significantly higher than those of fish fed CON diet, however, there were no significant differences among fish fed  the probiotic and OTC diets . B ased on these results, B. subtilis at 10 8 CFU/g ,  E. faecium at 10 7~8 CFU/g , and  L. lactis at 1 × 10 8 CFU/g could  be ideal probiotics in terms of the growth performance, immune responses, enzyme activity, disease resistance and gene expression, to replace  antibiotics in Japanese eel, Nile tilapia, and whiteleg shrimp.