Aquaculture America 2020

February 9 - 12, 2020

Honolulu, Hawaii

EFFECT OF DIFFERENT LEVELS OF DIETARY ?-AMINOBUTYRIC ACID (GABA) SUPPLEMENTATION ON JUVENILE OLIVE FLOUNDER Paralichthys olivaceus REARED AT TWO STOCKING DENSITIES

 
Wonsuk Choi*, Nathaniel W. Farris, Seonghun Won, Jinho Bae,  & Sungchul C. Bai
 Department of Marine Bio-materials and Aquaculture/Feeds and Foods Nutrition Research Center, Pukyong National University, Busan 608-737, Republic of Korea
E-mail: thm622@naver.com
 

Following a recent unpublished trial which determined the optimal dietary supplemental γ-Aminobutyric acid (GABA) level to be 145 mg kg-1, an 8-week feeding trial was conducted to determine the effects of GABA approximating optimal supplementation  at four different levels on juvenile olive flounder reared at both optimal and high stocking densities . A total of 1,080 fish with an average weight of 5.66 ± 0.02 g(mean±SD) were randomly stocked at two densities, 30 fish and 60 fish into 24, 40 L , 0.153 m2 seawater tanks which resulted in 1.11 and 2.22 kg/m2 respectively . Each t ank at both densities were  assigned to triplicates of four  dietary treatment groups; a basal diet without supplemental GABA (CON)  and three other diets prepared by adding 150 (GAB150) , 200 (GAB200), and 250 mg kg-1 (GAB250 ) GABA to the CON diet. Parameters including growth performance, non-specific immune responses , disease resistance, enzyme activity, histology, and genetic expression  are currently under analysis. The final r esults of this trial will be reported in conference.