An 4 -week feeding trial was conducted to evaluate the effects of dietary supplementation of enterococcus faecium as a probiotic and fructo-oligosaccharide (FOS) as a prebiotic in juvenile N ile tilapia , Oreochromis niloticus. Fish averaging 8.09 ± 0.21g (mean±SD ) were randomly distributed into seven groups of rectangular 45 L tanks (20 fish/tank) with three replicates , and e ach group of fish were fed by one of the seven experimental diets: A basal diet without probiotic or prebiotic supplementation was used as control (CON), and six other diets were formulated by adding 106 CFU/g E. faecium (EF6) , 107 CFU/g E. faecium (EF7) , 10 8 CFU/g E. faecium (EF8) , 106 CFU/g E. faecium + FOS (EF6F ), 107 CFU/g E. faecium + FOS (EF7F) and 10 8 CFU/g E. faecium + FOS (EF8F) to the CON diet. G rowth performance and other parameters would be measured after 4 weeks of feeding trial, and the results will be presented.