For development of prevention methods against shrimp bacterial infections diseases, we are studying several different ways such as feeding probiotic bacteria TOA5001, immune-stimulant 5-ALA (non protein amino acid residue), Immunoglobulin Y (IgY, chicken egg immunoglobulin), phage therapy, and nano-bubble technology.
We conducted challenge test with Vibrio parahaemolyticus AHPND strain, transcriptome analysis of shrimp, and metagenome analysis of microbial flora in shrimp stomach and intestine for studying their mechanisms of prevention against AHPND. We also studied anti-microbial activities of TOA5001. In case of IgY, we used recombinant AHPND toxins for immunization of chickens and used dried chicken egg powder as a feed additive for studying prevention of AHPND. We studied bacteriophage and ozone nano-bubble water for bactericidal activity. I will introduce our recent research results of these research topics. In addition, I also would like to introduce characterization of shrimp hemocytes for understanding shrimp immune system.