RNA/DNA RATIOS ALTERATION AND MEAT QUALITY EVALUATION OF FRESHWATER FISH COLLECTED FROM KOT KHAIRA, JHANG

The study was carried out to evaluate the alterations in RNA/DNA ratios and meat quality of freshwater fish. The fish was collected from drainage canal, Kot Khaira, Jhang. The study was approved by the ethical committee of Govt. College University, Faisalabad. Nucleic acids were extracted from the muscle tissues. The RNA/DNA ratios as  indicator of  growth were calculated by spectrophotometer at 600 nm. The meat quality was assessed by protein profiling using SDS-PAGE for qualitative assessment after quantitative analysis. All the data was analyzed statistically using the ANOVA.

The sampling of fish was carried out during September, 2018 to December, 2018. Control fish was procured from Fish seed hatchery, Satiana Road, Faisalabad. Water quality parameters of river water were assessed at the time of sampling using multi-parameter instrument HI-9829 HANNA meter throughout the experimental period.

The color of the sample is dark brownish to brown.The water sample has pungent smell which indicates presence of pollutants.The industrial waste water entering the river usually rises the surrounding temperature. The pH of the sample was 6.7.In the present water sample the D.O value was 1.29 milligram/liter.

After the dissection of fish the muscle piece was taken and nucleic acid i.e. DNA and RNA was isolated. The DNA and RNA values were estimated and difference in DNA and RNA concentrations and RNA/DNA ratio in different fish samples were observed.  It was observed that DNA & RNA concentrations were 0.093µg/ml & 0.238µg/ml, and 0.054µg/ml & 0.266µg/ml, in experimental & control fish respectively.

The RNA/DNA ratios showed the significant differences in all fish samples as compared with the control group (P<0.0001) The industrial effluents greatly influenced the meat quality of inhibiting that area. The protein quantity also showed significant difference..