In this study, a mixture of a suitable chalcone and p-hydrazinobenzenesulfonamide hydrochloride in 1:1.1 mol ratio in ethanol (25 ml) in the presence of glacial acetic acid (0.05 ml) was refluxed for synthesis of 4-(3-(4-Bromophenyl)-5-(2,4-dimethoxyphenyl)-4,5-dihydro-1H-pyrazol-1-yl) benzenesulfonamide, B4, for 7h at first experiment.
Effects of B4 on antioxidant enzymes of gill and liver, and changes in hematological parameters were determined in rainbow trout. For this purpose, rainbow trout alevins were exposed to 0, 0.25, 0.5, 1 and 2.5 mg/L of B4 and 1 ml of DMSO for 96 hours. Blood samples were collected for hematological analyzes and mortalities were recorded daily.
At the end of the study, it was determined that exposure to B4 has caused significant changes in antioxidant enzymes of gill and liver of alevins (p<0,05). Catalase (CAT) (EU/ mg protein), superoxide dismutase (SOD) (EU/mg protein) and glutathione peroxidase (GPx) (EU/mg protein) activity values reduced, while MDA values were increasing significantly (p<0.05) in gill and liver of fish compared to control group. SOD activity was time dependent, since it was reducing at initial and then increasing.
WBC, MCV, MCH and MCHC levels increased, while RBC Hb, Htc and PLT levels were decreasing by increasing the dose from 0.25 to 2.5 mg/L of B4 (p<0.05) compared to control. There were no differences between control and DMSO group (p>0.05) with respect to all tested properties.
It is suggested that B4 should be investigated further for possible usage in aquaculture industry to produce healthy fish.
Key words: CAT, SOD, GPx, rainbow trout, pyrazoline, B4