The present study was undertaken to assess the sub lethal toxic effects of carbofuran and profenofos on acetylcholinesterases (AChE) and butyrylcholinesterases (BuChE) in various tissues of Labeo rohita and was compared with control. The fingerlings of Cyprinus carpio (L= 92.5 ±6.3 mm, W= 33.44 ± 3.66 g) were exposed to three sub-lethal concentrations of profenofos (0.040, 0.020 and 0.013 mg/L) and carbofuran (0.199, 0.099 and 0.068 mg/L) in a three replicates for the total period of 60 days. The minimum AChE activity was recorded as 1.05 ± 0.02, 0.66 ± 0.02,1.91 ± 0.06,1.38 ± 0.24, 4.27 ± 0.03 and 1.69 ± 0.04 µmol/min/g protein in brain, gills, flesh, kidney, liver and blood, respectively with a dose of 0.040 mg / L of profenfos. The minimum BuchE activity was observed as 8.41 ± 0.06, 2.54 ± 0.31,1.91 ± 0.06, 4.41 ± 0.05,16.80 ± 0.05 and 8.69 ± 0.03 µmol/min/g protein in the brain, gills, flesh, kidney, liver and blood, respectively with a dose of 0.040 mg / L of carbofuran. The minimum AchE activity was recorded as 4.71 ± 0.05, 4.58 ± 0.04, 4.21 ± 0.14, 3.55 ± 0.12, 9.30 ±0.107 and 4.81 ± 0.04 µmol/min/g protein in brain, gills, flesh, kidney, liver and blood, respectively with exposure concentration of carbofuran (0.02 mg/L). The maximum BuChE activity was 13.75 ± 0.46, 2.56 ± 0.14, 3.91 ± 0.07, 7.40 ± 0.05,19.71 ± 0.08 and 6.82± 0.04 µmol/min/g protein in brain, gills, flesh, kidney, liver and blood, respectively with the exposure of 0.199 mg/L carbofuran. It has been concluded that profenofos was found to be the potent inhibitor of cholinesterases as compared to cabofuran.