As the United States production of warmwater marine cultured fish increases, the demand for good quality seed is increasing. To satisfy that increasing demand, spawning quality and seed production success must be improved. C ontrolling the nutritional quality of broodstock diets and first-feeding larvae . Identifying the appropriate essential dietary nutrients, especially in serial spawners with short vitellogenic periods, would greatly improve not only the egg quality but also seed production. Comparison of the biochemical composition of eggs and larvae, at different stages, and patterns of use and conservation of nutrients during early embryo and larvae development, are often used to study nutritional requirements of warmwater marine fish broodstock. Florida pompano (Trachinotus carolinus ) has been identified as a promising candidate for commercial scale aquaculture, but to date little information is available regarding captive broodstock dietary requirements. The present study aims to describe and compare lipid, fatty acid, and amino acid profiles in eggs and larvae from captive and wild Florida pompano throughout the reproductive season. The results to be presented will help evaluate the dietary fatty acids and amino acid requirements in captive broodstock in order to improve spawning and the production of high quality eggs.